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Semen Analysis in Urine | Retrograde Ejaculation

Monday, September 15, 2014
While the SQA-V does not have a specific mode for running retrograde ejaculation samples it is possible to run them using WASHED 20 MICRO mode a limited report of Motile Sperm Concentration, Progressive Motile Sperm Concentration, Velocity, and SMI.

For additional information on Retrograde Ejaculation, please find below a section of the WHO 5th ed. manual (p. 168) related to preparing Retrograde Ejaculation samples:



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MSDS Material Safety Data Sheet Updates for all MES Products

Thursday, August 21, 2014

The MSDS (Material Safety Data Sheets) Documents for all MES products have been updated.  Please find them HERE.

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Technical Bulletin Update | SQA-V Accessory Storage Conditions

Wednesday, August 20, 2014


The storage conditions for the SQA-V GOLD accessories and kits are provided with each product separately when/if controlled conditions are required and are specified in the Material Safety Data Sheet (MSDS) for each product. This technical bulletin provides an overall list of the SQA-V GOLD accessories storage conditions which are provided by the manufacturer.




See all current Technical Bulletins HERE.

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New Daily Maintenence Checklist Now Available for SQA Platforms

Tuesday, August 19, 2014

The Daily Maintenance Checklist for the SQA-V and all other SQA platforms has been updated to represent the new MES recommendations for cleaning (daily and weekly steps).  The new instructions can be downloaded in Excel format HERE.


Other related procedural documents can be found HERE.


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NEW SQA and QwikCheck Cleaning Instructions | Effective May 2014

Saturday, May 31, 2014
The SQA cleaning procedure has been updated for ease of maintenance and for system reliability. It was found that improper or excessive cleaning with more than one drop of cleaning fluid may impact the system over time. Please note that the new procedure differs from the previous version in the following three ways:

• Use of the 6” wooden electrostatic brush “long brush” is now STEP 1 and should be done DAILY.

• Use of the “blue dot cleaning paddle” is different, is STEP 2 and is performed WEEKLY (instead of daily)

• New cleaning kits will include the updated instructions and will have an outside label indicating a new kit.


UPDATED CLEANING KIT INSERT (click to download):




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SQA-V Service Manual Update | WHO 3rd 4th 5th Edition

Thursday, May 01, 2014

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Stabilized Sperm Proficiency Challenge for LOW LEVEL Target Values

Sunday, April 27, 2014



The need for external quality control schemes for confirming diagnostic procedures/equipment is of utmost importance. A number of multicentre studies on semen quality confirm this fact (1, 2). Quality control materials are best suited when they closely replicate the actual material being tested. However, due to the biological nature of semen, providing external quality control materials presents a challenge. Semen is motile for a relatively short period of time and the sample degenerates quickly. Semen samples prepared by proficiency/quality control organizations are based on adding a fixative media to the donor semen samples. As a result, the original nature of the fresh semen is distorted. In addition, there can be significant batch-to-batch variations in the proficiency challenge sample matrix. This causes problems running such samples in the SQA-V - especially at the low end of concentration.


Matrix and Spermatozoa Modification by Fixative Materials

Stabilized sperm is made from a fresh semen sample. This fixative process impacts the original semen sample as described below:


• The spermatozoa are shrunk because of fixation which causes a change to their size and shape.


• The seminal plasma is diluted with a fixative or completely replaced with a media causing a decrease in the sample viscosity. The SQA-V algorithm works based on the optical density of SEMINAL PLASMA (not the dilution media or modified “seminal plasma” used in stabilized sperm).


• A decrease in sample viscosity and density results in rapid cell sedimentation and uneven distribution of the cells throughout the volume of the sample. These factors may result in inconsistent concentration of cells in the samples received by the labs and in the aliquots taken for analysis. The sample tested may no longer reflect the target value established by the manufacturer.

All of these facts will adversely impact the accuracy of automated testing especially in low concentration samples because the prepared quality control material is not the same as a normal semen sample. The SQA-V is designed to run semen samples within one hour of collection and the concentration dynamic range of the SQA-V is 2 M/ml on the low end. Therefore, running a quality control sample of very low quality on the SQA-V, may result in a ZERO reading.


Running Low Level Stabilized Sperm Samples on the SQA-V GOLD System

If a stabilized sperm sample run on the SQA-V GOLD in the stabilized sperm Quality Control mode results in ZERO (beyond 2 M/ml dynamic range), re-run the sample in the Fresh mode as outlined below:

·        Turn on the SQA-V GOLD system and wait until auto-calibration/self-testing is completed.

·        Go to: MAIN MENU>TEST NEW PATIENT and enter:

o      PATIENT ID: Sample #

o      BIRTH DATE: Test date

·          From the next screen select:


o  WBC CONC: < 1 M/ml


·        Vortex the stabilized sperm sample.

·        Transfer the sample from the original vial to the 10-ml collection cup and mark it with the sample #.

·        Mix the sample thoroughly and immediately fill the SQA-V capillary and run the test.


1.      WHO laboratory manual for the examination and processing of human semen - 5th ed., World Health Organization, 2010.

2.      Auger J. et al. Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories, Human Reproduction, 2000, Vol. 15, Issue11, pp. 2360-2368.



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SQA-V Concentration Standard Explained

Friday, April 25, 2014
A number of commercially available counting chambers are used in laboratories for manually counting sperm cells world wide. These chambers vary by depth and some types require sample diluted while other do not.  It has been clinically established that counts can vary up to 30% depending on the type of chamber used.  The SQA-V permits the user to select the type of chamber the laboratory has implemented as a standard for manual semen analysis.  Once the concentration standard (CONC. STANDARD) has been selected the SQA-V will automatically run semen samples based on that standard.

Commercially available counting chambers are generally divided into two unique groups:

• Standard #1: 10-20 micron depth and do not require sample dilution.

• Standard #2: 100 micron depth (haemocytometers) that require sample dilution.


Please contact MES directly for additional questions about the type of chamber you use, and Remember, it ALL Started with a Sperm!

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WHO 5th Edition Manual STRICT Morphology Training and Testing

Tuesday, April 22, 2014


This document includes pages 67 through 95 of the WHO Laboratory Manual for the Examination and Processing of Human Semen FIFTH EDITION.  This updated version of the WHO manual does a very good job of showcasing normal vs. abnormal forms including all relevant defects.  Dr. Thinus Kruger assessed the slides presented personally and in addition to grading them provides comments to further explain his judgements.  It is highly recommended that all SQA-V users, and facilities conducting manual semen analysis become familiar with this new criteria as it is fast becoming the world standard.  The document also provides 12 plates worth of numbered cells which can be assessed by the operator for training and proficiency purposes (don't look at the answers ahead of time!)  The document can be downloaded by clicking the image above or by clicking HERE


Remember, it ALL Started with a Sperm.

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WHO Recommendations for Manual Sperm Concentration Analysis

Tuesday, April 22, 2014

Maintaining an accurate backup method for any automated analyzer in the laboratory is important.  When it comes to semen analysis, there are many different counting chamber options available.  Correlating manual methods to automated methods can be challenging, especially when it comes to semen analysis.  The first step is picking the correct tool for the job.  Below are the WHO 5th Edition Manual's recommendations for sperm concentration counting chambers.  To achieve the best possible correlation to the SQA-V Gold Automated Sperm Quality Analyzer, and maintain the most accurate backup method possible these recommendations should be followed.  In particular please note that disposable plastic counting chambers can lead to increased variability.  


2.7.1 Types of counting chambers (From Page 34 of the WHO 5th Edition Manual for Semen Analysis)

The use of 100-m-deep haemocytometer chambers is recommended. Dilution factors for the improved Neubauer haemocytometer chamber are given here. Other deep haemocytometer chambers may be used, but they will have different volumes and grid patterns and will require different factors for calculation. Disposable chambers are available for determining sperm concentration (Seaman et al., 1996; Mahmoud et al., 1997; Brazil et al., 2004b), but they may produce different results from those of the improved Neubauer haemocytometer. Shallow chambers that fill by capillary action may not have a uniform distribution of spermatozoa because of streaming (Douglas-Hamilton et al., 2005a, 2005b). It may be possible to correct for this (Douglas-Hamilton et al., 2005a) but it is not advised (Björndahl & Barratt, 2005). The validity of these alternative counting chambers must be established by checking chamber dimensions (see Appendix 7, section A7.8), comparing results with the improved Neubauer haemocytometer method, and obtaining satisfactory performance as shown by an external quality-control programme. For accurate assessment of low sperm concentrations, large-volume counting chambers may be necessary (see Section 2.11.2).

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