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Stabilized Sperm Proficiency Challenge for LOW LEVEL Target Values

Wednesday, October 29, 2014

Background:

 

The need for external quality control schemes for confirming diagnostic procedures/equipment is of utmost importance. A number of multicentre studies on semen quality confirm this fact (1, 2). Quality control materials are best suited when they closely replicate the actual material being tested. However, due to the biological nature of semen, providing external quality control materials presents a challenge. Semen is motile for a relatively short period of time and the sample degenerates quickly. Semen samples prepared by proficiency/quality control organizations are based on adding a fixative media to the donor semen samples. As a result, the original nature of the fresh semen is distorted. In addition, there can be significant batch-to-batch variations in the proficiency challenge sample matrix. This causes problems running such samples in the SQA-V - especially at the low end of concentration.

 

Matrix and Spermatozoa Modification by Fixative Materials


Stabilized sperm is made from a fresh semen sample. This fixative process impacts the original semen sample as described below:

 

• The spermatozoa are shrunk because of fixation which causes a change to their size and shape.

 

• The seminal plasma is diluted with a fixative or completely replaced with a media causing a decrease in the sample viscosity. The SQA-V algorithm works based on the optical density of SEMINAL PLASMA (not the dilution media or modified “seminal plasma” used in stabilized sperm).

 

• A decrease in sample viscosity and density results in rapid cell sedimentation and uneven distribution of the cells throughout the volume of the sample. These factors may result in inconsistent concentration of cells in the samples received by the labs and in the aliquots taken for analysis. The sample tested may no longer reflect the target value established by the manufacturer.


All of these facts will adversely impact the accuracy of automated testing especially in low concentration samples because the prepared quality control material is not the same as a normal semen sample. The SQA-V is designed to run semen samples within one hour of collection and the concentration dynamic range of the SQA-V is 2 M/ml on the low end. Therefore, running a quality control sample of very low quality on the SQA-V, may result in a ZERO reading.

 

Running Low Level Stabilized Sperm Samples on the SQA-V GOLD System

If a stabilized sperm sample run on the SQA-V GOLD in the stabilized sperm Quality Control mode results in ZERO (beyond 2 M/ml dynamic range), re-run the sample in the Fresh mode as outlined below:

·        Turn on the SQA-V GOLD system and wait until auto-calibration/self-testing is completed.

·        Go to: MAIN MENU>TEST NEW PATIENT and enter:

o      PATIENT ID: Sample #

o      BIRTH DATE: Test date

·          From the next screen select:

o  SAMPLE TYPE: FRESH

o  WBC CONC: < 1 M/ml

o  Select “Yes” when asked “IS SAMPLE VOLUME SUFFICIENT FOR COMPLETE RESTING >= .5 ml?

·        Vortex the stabilized sperm sample.

·        Transfer the sample from the original vial to the 10-ml collection cup and mark it with the sample #.

·        Mix the sample thoroughly and immediately fill the SQA-V capillary and run the test.

References

1.      WHO laboratory manual for the examination and processing of human semen - 5th ed., World Health Organization, 2010.

2.      Auger J. et al. Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories, Human Reproduction, 2000, Vol. 15, Issue11, pp. 2360-2368.

 

DOWNLOAD THIS TECHNICAL BULLETIN HERE



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Importing the SQA-V Archive into V-Sperm

Tuesday, October 14, 2014

 

This training video provides instructions on how to import the SQA-V archive into V-Sperm. Periodically, the SQA-V archive may become full generating a message prompting your to clear it. Follow this guide to make that happen! Remember, it ALL Started with a Sperm!  See additional videos HERE



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SQA-V Cleaning Procedure Update | Daily and Weekly Maintenance

Tuesday, October 07, 2014

 

As of mid 2014 the SQA-V cleaning procedure has been broken up into daily steps and weekly steps for ease of maintenance and to avoid excessive system cleaning. Keep follow these steps to keep your system reliable, accurate, and happy! Remember, it ALL Started with a Sperm!  See additional videos HERE



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Changing the SQA-V Gold Printer Ribbon | Training Video

Wednesday, October 01, 2014

 

This demonstration video showcases the changing of the SQA-V printer ribbon.  Watch closely and Remember, it ALL Started with a Sperm!  See more training videos HERE



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Using the SQA-V and V-Sperm Visualization System

Tuesday, September 30, 2014

 

This demonstration video provides an overview on the use and function of the SQA-V / V-Sperm Visualization System. Used together, the system allows the user to view and record videos, take pictures of the semen sample, and accurately count using the system's slide adapter. Remember it ALL Started with a Sperm! See more training videos HERE



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V-Sperm Gold PC Computer Minimum Requirements Overview | Click Image

Monday, September 22, 2014


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Semen Analysis in Urine | Retrograde Ejaculation

Monday, September 15, 2014
While the SQA-V does not have a specific mode for running retrograde ejaculation samples it is possible to run them using WASHED 20 MICRO mode a limited report of Motile Sperm Concentration, Progressive Motile Sperm Concentration, Velocity, and SMI.

For additional information on Retrograde Ejaculation, please find below a section of the WHO 5th ed. manual (p. 168) related to preparing Retrograde Ejaculation samples:

 

 



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MSDS Material Safety Data Sheet Updates for all MES Products

Thursday, August 21, 2014


The MSDS (Material Safety Data Sheets) Documents for all MES products have been updated.  Please find them HERE.


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Technical Bulletin Update | SQA-V Accessory Storage Conditions

Wednesday, August 20, 2014
BACKGROUND:

 

The storage conditions for the SQA-V GOLD accessories and kits are provided with each product separately when/if controlled conditions are required and are specified in the Material Safety Data Sheet (MSDS) for each product. This technical bulletin provides an overall list of the SQA-V GOLD accessories storage conditions which are provided by the manufacturer.

 

DOWNLOAD THIS TECHNICAL BULLETIN

 

See all current Technical Bulletins HERE.



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New Daily Maintenence Checklist Now Available for SQA Platforms

Tuesday, August 19, 2014

The Daily Maintenance Checklist for the SQA-V and all other SQA platforms has been updated to represent the new MES recommendations for cleaning (daily and weekly steps).  The new instructions can be downloaded in Excel format HERE.

 

Other related procedural documents can be found HERE.

 



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