Rapid - Objective - Standardized Semen Analysis Solutions... Remember, it ALL Started with a Sperm!
In addition to the obvious issues with sample loading in the SQA testing capillary, would concentration readings be higher or lower in a sample which has not fully liquefied?
Based on the required semen sample testing volume of 0.5 mL, it is recommended to fill the entire testing capillary with a semen sample without any dilution if the ejaculate volume is >= 0.5 mL. Sometimes though the ejaculate volume may be less than 0.5 mL. The dilution mode of the SQA-V and Vision can be used in this case as follows: If the semen sample volume is >= 0.3 mL, dilute the sample using the QwikCheck Dilution media two times (1:2 or 1+1). For example, 0.3 mL of semen sample is mixed with 0.3 mL of diluent resulting in a volume of 0.6 mL. This will provide an adequate sample volume to aspirate into the SQA testing capillary and the SQA software automatically compensates for the dilution and will provide a full report, no calculations are required by the operator.
Based on the sample volume, M.E.S. recommends the following approach to testing the sample:
• Semen Volume >= 0.5 mL - No dilution is required
• Semen Volume >= 0.3 mL and < 0.5 mL - Dilution 1:2 (1+1) using the QwikCheck Dilution kit
• Semen Volume < 0.3 mL - Manual assessment [microscope or Vision Manual Mode or Vision Semi-automated Mode (10 µL automated testing + 10 µL manual motility assessment)]
• WHO laboratory manual for the examination and processing of human semen - 5th ed., WHO 2010.
• SQA-V GOLD User Guide Version 2.60 I-Button
• SQA-Vision User Guide Version 104.13.2
TECHNICAL BULLETIN: TESTING CAP PROFICIENCY SAMPLES ON SQA SEMEN ANALYZERS
For All SQA-V and SQA-VISION Systems Version 2.45 and above | UPDATE: April 27th, 2016
The College of American Pathologists (www.cap.org) provides a nationwide proficiency challenge for automated methods of semen analysis. These results are peer reviewed against other SQA users providing an objective and efficient way to maintain proficiency. MES recommends this survey as an unbiased appraisal of user proficiency and system performance.
TESTING INSTRUCTIONS FOR SQA-V/SQA-V GOLD/SPERMALITE SYSTEMS:
NOTE: The CAP proficiency material should first be run as Stabilized Sperm in the “Control” mode and re-run in the Test New Patient “Fresh” Mode if a result of 0.0 is reported in Stabilized Sperm Control mode.
SETTING UP THE DEFAULTS:
TESTING YOUR PROFICIENCY SAMPLES ON THE SQA-V/SQA-V Gold/Spermalite:
TESTING INSTRUCTIONS FOR SQA-VISION SYSTEMS:
SETTING UP THE DEFAULTS:
TESTING YOUR PROFICIENCY SAMPLES ON THE SQA-VISION:
Click the image below for a screen shot overview of the SQA-VISION instrument. The document may be used as a training reference tool for MES distributors and end users looking to train new staff members. Remember, it ALL Started with a Sperm!
Answer: Leukocytes, predominantly polymorphonuclear leukocytes (PMN, neutrophils), are present in most human ejaculates (Tomlinson et al., 1993; Johanisson et al., 2000). They can sometimes be differentiated from spermatids and spermatocytes in a semen smear stained with the Papanicolaou procedure. Differentiation is based on differences in staining coloration, and on nuclear size and shape (Johanisson et al., 2000). Polymorphonuclear leukocytes can easily be confused morphologically with multinucleated spermatids, but stain a bluish colour, in contrast to the more pinkish colour of spermatids (Johanisson et al., 2000). Nuclear size may also help identification: monocyte nuclei exhibit a wide variation in size, from approximately 7 µm for lymphocytes to over 15 µm for macrophages. These sizes are only guidelines, since degeneration and division affect the size of the nucleus. There are several other techniques for quantifying the leukocyte population in semen. As peroxidase-positive granulocytes are the predominant form of leukocytes in semen, routine assay of peroxidase activity is useful as an initial screening technique (Wolff, 1995; Johanisson et al., 2000) / (WHO 5th ed. manual, p. 102).
For testing pH and WBC in semen, Medical Electronic Systems (www.mes-global.com) provides FDA cleared QwikCheck™ Test Strips which are specifically labeled for semen sample testing. Customers have requested a comparison of the QwikCheck™Test Strips to urine Test Strips. Please find a link to the comparison below:
Note: False positive results may occur when using urine test strips (for semen samples with WBC ≥ .5M/ml and ≤ 1M/ml (as the upper range for Urine test strips is .5M/ml.
Question - What goes into the calculation of "Sperm Per Scan" on the Post Vasectomy mode of the SQA-V Automated Sperm Quality Analyzer?
Answer - The # Motile Sperm Per Scan is assessed automatically by the SQA-V algorithm based on detections of electronic signal spikes generated by the system when sperm cells cross the light beam in the thin section of the capillary (~ 10 µL volume, area 33mm2, 300µm depth). To compare to manual semen assessment, the "high power field" (x400) under the microscope using a standard slide contains 0.0024 µL of semen (area 0.12 mm2 X depth 0.02 mm). The total number of motile spermatozoa detected automatically in a 5-minute testing cycle is reported as # Motile Sperm Per Scan.